putative rflp analysis between hsvd-sb and closely related variant

نویسندگان

saa bagherian department of horticultural sciences, college of agriculture, jahrom university, jahrom, iran.

چکیده

sweet lime split bark is a disorder of increasing importance in fars province. previously we showed that a new variant of hop stunt viroid (hsvd-sb) was related to the disorder. it had homology with a noncachexia variant of hop stunt viroid (cvd-iia-117) which causes cracking the bark of trifoliate orange rootstocks. in this study, showed that m.ngobix, bgli and bsri restriction enzymes can cut hsvd-sb according to putative rflp analysis using vector nti program (version 9.0.0) database but these restriction enzymes can’t cut cvd-iia-117. in other hand some restriction enzymes can cut cvd-iia-117 but can’t cut hsvd-sb. therefore these two variants can be recognized by these restriction enzymes.

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عنوان ژورنال:
iranian journal of virology

جلد ۸، شماره ۱، صفحات ۳۳-۳۴

کلمات کلیدی
[ ' h s v d ' , ' s b ' , ' c v d ' , ' i i a ' , 1 1 7 , ' r e s t r i c t i o n f r a g m e n t l e n g t h p o l y m o r p h i s m a n a l y s i s ' ]

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